Scientific activity

Areas of scientific activity

  • Biophysical analysis of specific intermolecular interactions of proteins and ribonucleic acid (RNA) fragments in translation, mRNA splicing and intracellular transport.
  • Chemical syntheses and biological functionality in gene expression, of oligonucleotide mRNA 5' cap analogues.
  • Development and application of relaxation, and computer symulation, methods, in structural, dynamic, and functional studies of biopolymrers and characteristic of some selected biomolecular systems.
  • Development and application of large-scale molecular modelling and bioinformatics.
  • Identification and characteristics of the ligand structures in enzyme active centres by emission spectroscopy; mechanisms of enzymatic activity of purine nucleoside phosphorylases and thymidilate synthases.
  • Structures and spectral properties of plant thylacoids.
  • Biophysical basis of purine nucleoside phosphorylases activity; synteses and biological studies of the inhibitors of potential chemiotherapeutic activities.

Experimental techniques

  • molecular spectroscopy (UV-VIS, fluorescence emission, stopped-flow, NMR, CD), X-ray diffraction,
  • calorimetry,
  • mass spectrometry,
  • molecular photophysics,
  • electrochemistry, chemistry,
  • enzymology,
  • genetic engineering.

Theoretical methods

  • classical, Brownian, and quantum molecular dynamics,
  • molecular modeling,
  • multiscale modelling and bioinformatics,
  • quantum chemistry.

Laboratories and equipment

Standard biophysical laboratory instruments and modern equipment purchased from the POIG projects:

  • Innovative Economy Operational Programme Priority 2. Action 2.1. POIG.02.01.00-14-122/09. Physics as the basis for new technologies - development of modern research infrastructure at the Faculty of Physics of the University of Warsaw - Biopolymers Laboratory
  • Innovative Economy Operational Programme, Priority 2. Action 2.2. POIG.02.02.00-00-025/09. Support of Formation of Common Research Infrastructure of Scientific Units. National Multidisciplinary Laboratory of Functional Nanomaterials - Analytical Ultracentrifugation Laboratory

Biopolymers Laboratory

Analytical Ultracentrifugation Laboratory

Research groups and projects

Studies of the macromolecular structure, dynamics and function in solution

Purine nucleoside phosphorylases (PNP): three-dimensional structure and molecular mechanism of catalysis

Role of 5' mRNA and 5' U snRNA cap structures in regulation of gene expression